(I)
40% formamide
10% dextran sulfate
1 x Denhardt’s
4 x SSC
10 mM DTT
1 mg/ml tRNA
1 mg/ml salmon sperm DNA
(II)
2 x SSC
10 % dextran sulfate
0.01 % salmon DNA
0.02 % SDS
50 % formamide
(III)
50 % formamide
2.25 x SSPE (300 mM NaCl)
10 % dextran sulfate
2.5 x Denhaldt’s
0.1 mg/ml tRNA and salmon DNA
5 mM DTT
(IV)
4 x SSC
10 % dextran sulfate
1 x Denhardt’s
2 mM EDTA
50 % formamide
0.5 mg/ml salmon DNA
(V)
50 % formamide
5 x SSC
50 μg/ml heparin
0.1 % Tween 20
0.1 mg/ml salmon DNA
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